Four Lincolnshire MPs voted against gay marriage despite the Marriage (Same Sex Couples) Bill getting the backing of the House of Commons last night.
The Bill had 400 MPs voting in favour and 175 against, a majority of 225.
Lincoln's Conservative MP Karl McCartney voted against, as did Sir Peter Tapsell, MP for Louth and Horncastle.
John Hayes, MP for South Holland and the Deepings and Gainsborough MP Edward Leigh, who spoke during the Commons debate before the vote, also went against Prime Minister David Cameron.
During the debate, Mr Leigh said: "We should indeed treat one another with tolerance and treat everybody's sexuality with understanding, but the fundamental question we are deciding today is whether English law should declare for the first time that two people of the same sex can marry.
"Parliament is sovereign—we can vote for what we want—but we must be very careful that law and reality do not conflict.
"In 1648, the Earl of Pembroke, in seeking to make the point that Parliament is sovereign, said that Parliament can do anything but make a man a woman or a woman a man.
"Of course, in 2004, we did exactly that with the Gender Recognition Act.
"We are now proposing to make equally stark changes to the essence of marriage.
"During the civil partnership debates, I was given solemn assurances on the Floor of the House, including by some sitting on the Opposition Benches now, that the Civil Partnership Act would not lead to full same-sex marriage."
Sleaford and North Hykeham MP Stephen Phillips was not present for the vote, but intends to vote in favour at the third reading stage.
Only Nick Boles, Grantham and Sleaford MP and Mark Simmonds, MP for Boston and Skegness, voted in favour of the Bill.
Meanwhile, a group of University of Lincoln societies, including Liberal Youth, The Amnesty Society and Labour Students, as well as members of the local community, will be marking the passing of the Bill with a day of celebrations.
They will be meeting in Castle Square, at the top of Steep Hill, for a flash-mob that will involve facepainting, singing and dancing before moving down the High Street to the University of Lincoln for speeches.
It will start at 1.30pm on February 9.